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US 6,387,633 B1Granted
Method for identifying unstable genes
AssigneeSupratek Pharma Inc.
InventorAnatoli Onichtchenko
Life of this patent
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Abstract
The present invention provides a method for the generation and identification cell lines that posses genetically unstable genes. More specifically, the present invention provides a method for detecting genes associated in genetic alterations by using specific genes capable of inducing genetic instability such as metastasin 1 gene and their protein products.
Claims6
A method for identifying an unstable gene that is involved in genomic instability in cells, comprising the steps of:
- (a) transfecting the cells with a vector expressing human mts-1 or a functional analog thereof;
- (b) selecting for the transfected cells that express human mts-1 or the functional analog;
- (c) subcloning the cells resulting from step (b);
- (d) comparing the phenotypes of the subclones resulting from step (c);
- (e) determining a correlation coefficient for each gene in a gene panel by comparing the gene dosage of each gene in the gene panel with the gene dosage of a marker gene in genomic DNA isolated from the subclones;
- and
- (f) identifying a gene in the gene panel as an unstable gene if the phenotypes of the subclones demonstrate diversity and the correlation coefficient of the gene in the gene panel is less than 0.3.
4 more elements
A method for identifying an unstable gene that is involved in genomic instability in cells, comprising the steps of:
- (a) transfecting the cells with a vector expressing human mts-1 or a functional analog thereof, which vector expresses at least one other gene;
- (b) selecting for the transfected cells that express human mts-1 or the functional analog and which express the other gene;
- (c) subcloning the cells resulting from step (b);
- (d) comparing the phenotypes of the subclones resulting from step (c);
- (e) determining a correlation coefficient for each gene in a gene panel by comparing the gene dosage of each gene in the gene panel with the gene dosage of a marker gene in genomic DNA isolated from the subclones;
- and
- (f) identifying a gene in the gene panel as an unstable gene if the phenotypes of the subclones demonstrate diversity and the correlation coefficient of the gene in the gene panel is less than 0.3.
4 more elements
Dependencies are inferred from the claim text.