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US 6,387,632 B2Granted
Polynucleotide separation method and apparatus therefor
AssigneeHitachi, Ltd.
InventorsKenji Yasuda, Kazunori Okano, Hirokazu Kato
Life of this patent
Estimating the term…
Abstract
Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.
Claims4
A method for recovering a target polynucleotide in a cell comprising the steps of:
- (1) supplying a sample solution containing cells each containing polynucleotides and proteins on surface of a substrate which is disposed in a separation cell, and capturing each of said cells on each of a plurality of independent areas formed on the surface of the substrate having single-stranded oligonucleotide probes each having a specified base sequence immobilized to each of the plurality of independent areas;
- (2) replacing said sample solution on said substrate with a solution containing no polynucleotide, wherein said solution has a pH value of 4 or lower;
- (3) heating the surface of the substrate at one area of the plurality of independent areas to a first predetermined temperature to destroy said cell captured at said one area, to liberate said polynucleotides and said protein from said cell captured at said one area, and to denature polynucleotides so as to obtain single-stranded polynucleotides;
- (4) cooling said solution to a second predetermined temperature to form hybrids between single-stranded polynucleotides and said single-stranded olignucleotide probes, so as to capture single-stranded target polynucleotides;
- (5) separating said single-stranded target polynucleotides from said proteins, while said hybrids remain on said one area, by electrophoresis under DC field applied between a first electrode in said one area as an anode and a second electrode opposite to the first electrode as a cathode, based on a charge difference between said single-stranded target polynucleotides and said proteins, in said solution having a value of pH being 4 or lower;
- (6) recovering said proteins from said separation cell, by flowing a washing solution in to said separation cell, while said cells at the areas except for said one area remain on the areas and said hybrids remain on said one area;
- (7) separating said single-stranded polynucleotides not forming said hybrids from said hybrids, while said hybrids remain on said one area, by electrophoresis under DC field applied between the first electrode in said one area as the cathode and the second electrode opposite to the first electrode as the anode;
- (8) recovering said single-stranded polynucleotides not forming said hybrid from said separation cell, by flowing the washing solution into said separation cell;
- (9) heating the surface of the substrate at said one area of the plurality of independent areas to denature said hybrids at said one area, so as to liberate said single-stranded target polynucleotides into solution;
- (10) recovering said liberated single-stranded target polynucleotides from said separation cell;
- by flowing the washing solution into said separation cell;
- and
- (11) repeating the steps (3) to (10), by changing a position of said one area of the plurality of independent areas.
10 more elements
A method for recovering a target polynucleotide in a cell comprising the steps of:
- (1) supplying a sample solution containing cells each containing polynucleotides and proteins on a surface of a substrate which is disposed in a separation cell, and capturing each of said cells on each of a plurality of independent areas formed on the surface of the substrate having single-stranded oligonucleotide probes each having a specific base sequence immobilized to each of the plurality of independent areas;
- (2) replacing said sample solution on said substrate with a solution containing no polynucleotide, wherein said solution has a pH value of 4 or lower;
- (3) destroying said cell captured at said one area of the plurality of independent areas to liberate said polynucleotides and said proteins from said cell captured at said one area;
- (4) forming hybrids between single-stranded polynucleotides generated in said solution and said single-stranded oligonucleotides probes, so as to capture single-stranded target polynucleotides;
- (5) separating said single-stranded polynucleotides from said proteins, while said hybrids remain on said one area, by electrophoresis under DC field applied between a first electrode in said one area as an anode and a second electrode opposite to the first electrode as a cathode, based on a charge difference between said single-stranded polynucleotides and said proteins, in said solution having a value of pH being 4 or lower;
- (6) recovering said proteins from said separation cell, by flowing a washing solution into said separation cell, while said cells at the areas except for said one area remain on the areas and said hybrids remain on said one area;
- (7) separating said single-stranded polynucleotides not forming said hybrids from said hybrids, while said hybrid remain on said one area, by electrophoresis under DC field applied between the first electrode in said one area as the cathode and the second electrode opposite to the first electrode as the anode;
- (8) recovering said single-stranded polynucleotides not forming said hybrid from said separation cell, by flowing the washing solution into said separation cell;
- (9) heating the surface of the substrate at said one area of the plurality of independent areas to denature said hybrids at said one area, so as to liberate said single-stranded target polynucleotides into solution;
- (10) recovering said liberated single-stranded target polynucleotides from said separation cell;
- by flowing the washing solution into said separation cell;
- and
- (11) repeating the steps (3) to (10), by changing a position of said one area of the plurality of independent areas.
10 more elements
Dependencies are inferred from the claim text.