Polymer coated surfaces for microarray applications
AssigneeIncyte Genomics, Inc.
InventorsLyle J. Arnold, Samuel P. Sawan, Paul H. Lee
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Abstract
Methods are provide for modifying a solid support, such as a glass slide, by silylating with an agent having the formula H2N—(CH2)n—SiX3 where n is between 1 and 10, and X is independently chosen from OMe, OEt, Cl, Br, or I, then activating with a crosslinking reagent, followed by reacting with an amine-containing polymer. The support can optionally be reacted with a crosslinking reagent again. The support thus modified may be used to make arrays and microarrays where a plurality of targets are stably associated with the support and arranged in a defined manner.
Claims34
A method of attaching target molecules to a surface of a solid support, the method comprising the ordered steps of:
- a) silylating the surface with an agent having the formula H2N—(CH2)n—SiX3 where n is between 1 and 10, and X is independently chosen from OMe, OEt, Cl, Br, or I,
- b) activating the surface with a first crosslinking reagent;
- c) reacting the surface with an amine-containing polymer to form a modified solid support surface;
- and
- d) attaching non-activated target molecules to the modified solid support surface, wherein the target molecules are arranged in a defined manner on the modified solid support surface thereby producing an array of attached target molecules.
Dependencies are inferred from the claim text.