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US 6,387,626 B1Granted
Covalent attachment of unmodified nucleic acids to silanized solid phase surfaces
AssigneeOrchid BioSciences, Inc.
InventorsJufang Shi, Michael T. Boyce-Jacino
Life of this patent
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Abstract
The invention relates to a simple, cost effective method for immobilizing synthetic nucleic acid molecules onto a solid support The invention further concerns the use of such immobilized molecules in nucleic acid hybridization assays, sequencing by hybridization assays, and genetic analyses and combinatorial analyses involving nucleic acids or proteins for screening applications.
Claims27
A method for immobilizing an unmodified nucleic acid molecule to a solid phase, which comprises the steps of:
- (A) coating said solid phase with a silane and allowing said silane-coated solid phase to cure;
- and
- (B) coupling unmodified nucleic acid molecules having either a terminal 3′ OH or a terminal 5′ OH to said silane-coated solid phase.
Dependencies are inferred from the claim text.