Methods for using redox liposome biosensors
AssigneeThe Regents of the University of California
InventorsQuan Cheng, Raymond C. Stevens
Life of this patent
Estimating the term…
Abstract
The present invention provides methods and compositions for detecting the presence of biologically-important analytes by using redox liposome biosensors. In particular, the present invention provides liposome/sol-gel electrodes suitable for the detection of a wide variety of organic molecules, including but not limited to bacterial toxins.
Claims19
A method for measuring biomolecular recognition of at least one toxin by electrochemistry, comprising:
- a) providing:
- i) an electrode,
- ii) an insulator, and
- iii) liposomes having oxidation/reduction receptors, wherein said liposomes comprise a glycine-terminated diacetylene lipid, an acetylferrocenic diacetylene lipid comprising ferrocene, and a glycosphingolipid, and wherein said glycosphingolipid is known to be a receptor for at least one toxin;
- b) adding a sample suspected of containing at least one toxin to said liposomes;
- and
- c) measuring the current through said ferrocene to determine the biomolecular recognition of said at least one toxin by said liposomes, wherein a drop in said current is indicative of specific biomolecular recognition.
A method for measuring biomolecular recognition of a toxin by electrochemistry, comprising:
- a) providing:
- i) an electrode,
- ii) an insulator,
- iii) liposomes having oxidation/reduction receptors, wherein said liposomes comprise a glycine-terminated diacetylene lipid, an acetylferrocenic diacetylene lipid comprising ferrocene, and a glycosphingolipid, and wherein said glycosphingolipid is known to be a receptor for the 84-kDa E. coli enterotoxin, and
- iv) a sample suspected of containing E. coli enterotoxin;
- b) adding said sample to said liposomes;
- and
- c) measuring the current through said ferrocene to determine the biomolecular recognition of said E. coli enterotoxin by said liposomes, wherein a drop in said current is indicative of specific biomolecular recognition.
A method for measuring biomolecular recognition of an analyte by electrochemistry, comprising:
- a) providing:
- i) an electrode,
- ii) an insulator,
- iii) liposomes having oxidation/reduction receptors, wherein said liposomes comprise a glycine-terminated diacetylene lipid, an acetylferrocenic diacetylene lipid comprising ferrocene, and a glycosphingolipid, and wherein said glycosphingolipid is known to be a receptor of an analyte, and
- iv) a sample suspected of containing at least one analyte;
- b) adding said sample to said liposomes, and
- c) measuring the current through said ferrocene to determine the biomolecular recognition of the analyte by said liposomes, wherein a drop in said current is indicative of specific biomolecular recognition.
Dependencies are inferred from the claim text.