Compositions containing at least one nucleic acid
AssigneeCapsulis
InventorsPatrick Mahy, Didier Roux, René Laversanne, Joëlle Amedee, OLivier Freund
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Abstract
A composition containing a plurality of multicellular vesicles and at least one nucleic acid, at least one part of which is found included inside the multilamellar vesicles. Each of the vesicles is formed of a succession of lamellar bi-layers extending from each vesicle center to its periphery and including at least one surfactant agent. The bi-layers are concentric and are separated by a liquid medium. Such compositions can be used in pharmaceutics, particularly gene therapy, and in in vitro and in vivo transfection.
Claims24
A composition containing a plurality of multilamellar vesicles and at least one nucleic acid, at least one part of which is found included inside said multilamellar vesicles, wherein said vesicles are obtained by rearrangement of a lamellar liquid crystal phase comprising at least one surfactant and incorporating at least one nucleic acid by shearing or mechanical stirring, and wherein each said vesicle comprises a succession of lamellar bi-layers extending from each vesicle center to its outermost layer and comprising at least one surfactant agent, said bi-layers being concentric and separated by a liquid medium.
In a method for delivery of a nucleic acid to a cell to alter a cell function or to cause a new cell function, the improvement comprising delivery to the cell of a composition containing a plurality of multilamellar vesicles and at least one said nucleic acid, at least part of which is found included inside said multilamellar vesicles, wherein each said vesicle comprises a succession of lamellar bi-layers extending from each vesicle center to its periphery and comprising at least one surfactant agent, said bi-layers being concentric and separated by a liquid medium.
Dependencies are inferred from the claim text.