N-[1, (1-1) -dialkyloxy] - and N- [1, (1-1) -dialkenyloxy]- alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
OwnerDeborah A. Eppstein, Philip L. Felgner +3inventors
InventorsDeborah A. Eppstein, Philip L. Felgner, Thomas R. Gadek, Gordon H. Jones, Richard B. Roman
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Abstract
This invention relates to compounds of the formula or an optical isomer thereof wherein R1 and R2 are the same or different and are an alkyl or alkenyl group of 6 to 24 carbon atoms; R3, R4 and R5 are the same or different and are alkyl of 1 to 8 carbon atoms, aryl, aralkyl of 7 to 11 carbon atoms, or when two or three of R3, R4, and R5 are taken together to form quinuclidino, piperidino, pyrrolidino, or morpholino; n is 1 to 8; and X is a pharmaceutically acceptable anion.
Claims26
A method for introducing nucleic acids into mammalian cells which method comprises forming liposomes from positively charged lipids, contacting the liposomes with the nucleic acids to form nucleic acid-liposome complexes, wherein the nucleic acids are not encapsulated within the aqueous phase of the liposomes, and contacting the complexes with the mammalian cells under in vivo or in vitro conditions which are conducive to the transfection of the mammalian cells.
A method for transfecting mammalian cells with polynucleotides which method comprises forming liposomes from positively charged lipids, complexing the liposomes with the polynucleotides to form polynucleotide-liposome complexes, wherein the polynucleotides are not encapsulated within the aqueous phase of the liposomes, and contacting the complexes with mammalian cells, under in vivo or in vitro conditions which are conducive to transfection of the cells.
A method for transfecting mammalian cells with polynucleotides which method comprises contacting the mammalian cells with polynucleotide-liposome complexes, under in vivo or in vitro conditions which are conducive to transfection, said liposome formed from positively charged lipids, said polynucleotides not encapsulated within the aqueous phase of the liposomes.
Dependencies are inferred from the claim text.