US 6,387,268 B1Granted

Immobilization of membrane receptor on HPLC

AssigneeRett Corporation

InventorsIrving Wainer, Yanxiao Zhang

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Abstract

This invention provides a immobilized receptors on supports in liquid chromatographic systems. The method of the invention provides means of evaluating the attachment of agents to receptors comprising the steps of: (a) immobilizing receptors on artificial membrane supports in a column, (b) exposing the supports with the receptors to test agents at varying concentrations in a liquid chromatographic system, (c) eluting the test agent from the column, and (d) evaluating the elution profile of the test materials from the column. Using this method, it is possible to evaluate the interaction of the test agent with the receptor. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.

Claims13

Dependent claims

A method of identifying whether a compound is a specific ligand for at least one cellular receptor comprising (1) contacting an artificial membrane support having non-covalently immobilized thereon, said at least one cellular receptor to a liquid flow system comprising said compound,

and (2) determining whether said compound becomes specifically bound to said support wherein said cellular receptor is immobilized such that its conformational structure after immobilization permits specific binding to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment, said artificial membrane support is contained in a liquid flow system;
and said artificial membrane support is produced by the following steps:
(i) obtaining an immobilized artificial membrane (IAM) liquid chromatographic (LC) stationary phase comprising a phospholipid monolayer;
(ii) contacting said IAM LC stationary phase with at least one solubilized receptor or receptor-ligand output under conditions wherein that at least one solubilized receptor or receptor-ligand output becomes non-covalently immobilized in the phospholipid monolayer of said IAM LC stationary phase such that the tertiary structure of said at least one immobilized cellular receptor permits specific binding to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment;
and
(iii) contacting said artificial membrane support comprising at least one non-covalent immobilized cellular receptor to a liquid flow system.

3 more elements

A method for identifying, purifying or isolating a compound that affects the binding of a ligand to a cellular receptor that is specifically bound by said ligand comprising:

(i) contacting an artificial membrane support having non-covalently immobilized thereon at least one cellular receptor and to which at least one cellular receptor is itself specifically bound to a ligand to a liquid flow system that contains at least one compound that potentially affects the binding of said ligand which is specifically bound to said non-covalently immobilized cellular receptor;
(ii) identifying, purifying or isolating said at least one compound based on its ability to affect the binding of said ligand to said immobilized cellular receptor;
wherein said immobilized cellular receptor is immobilized to said artificial membrane support such that after immobilization specifically binds to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment, and wherein said artificial membrane support is contained in a liquid flow system;
and further wherein said artificial membrane support is produced by the following steps:
(1) obtaining an immobilized artificial membrane (IAM) liquid chromatographic (LC) stationary phase comprising a phospholipid monolayer;
(2) contacting said IAM LC stationary phase with at least one solubilized receptor or solubilized receptor-ligand complex under conditions wherein that at least one solubilized receptor or solubilized receptor-ligand complex becomes non-covalently immobilized in the phospholipid monolayer of said IAM LC stationary phase such that at least one immobilized cellular receptor permits specific binding to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment;
and
(3) optionally contacting said artificial membrane support to which is immobilized at least one cellular receptor with at least one ligand that specifically binds said at least one immobilized receptor under conditions that result in said ligand becoming specifically bound to said at least one cellular receptor non-covalently immobilized to said support.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/619,505Filed 19 Jul 2000 · division
Priority: 23 Feb 1998
Granted: 14 May 2002
Term ends: Not estimatedThe source records don’t support an estimate.

Classification18 codes

B01D 15/3804
Involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or…
B01D 15/1896
Relating to flow patterns: membrane chromatography or membrane adsorbers
B01J 20/28033
Characterised by their form: membrane, sheet, cloth, pad, lamellar or mat
B01J 20/286
Sorbents specially adapted for preparative, analytical or investigative chromatography: phases chemically bonded to a substrate, e.g. to silica or…