Method of purifying statins from a fermentation broth
AssigneeBiogal Gyogyszergyar Rt.
InventorsVilmos Keri, Lajos Deak, Iiona Forgacs
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Abstract
A process for purifying statin compounds from a fermentation broth by extraction and crystallization is disclosed. A fermentation broth is subjected to a pretreatment procedure which involves an alkaline pretreatment and an alkaline purification. Following the pretreatment procedure, the statin compound is extracted under acidic conditions into a hydrophobic solvent and purified by crystallization. The organic extraction solvent is concentrated and then extracted with a mild base. The statin compound is then purified by crystallization.
Claims29
A process for isolating a statin compound from a fermentation broth, wherein the statin compound comprises a carboxylic acid capable of forming a lactone and a fused bicyclic ring, comprising the steps of:
- (a) pretreating the fermentation broth under alkaline conditions to remove non-polar impurities;
- (b) extracting the statin compound from the fermentation broth by contacting the fermentation broth with an extraction solvent, and extracting the compound from the fermentation broth into the extraction solvent, wherein the extraction solvent is a hydrophobic organic extraction solvent;
- (c) separating the hydrophobic organic extraction solvent from the fermentation broth;
- (d) concentrating a solution of the hydrophobic organic extraction solvent containing the extracted statin compound;
- and
- (e) purifying the extracted statin compound by crystallization.
A process for isolating a statin compound from a fermentation broth, wherein the statin compound comprises a carboxylic acid capable of forming a lactone and a fused bicyclic ring, the process comprising the steps of:
- (a) pretreating the fermentation broth under alkaline condition to remove non-polar impurities;
- (b) extracting the statin compound from the fermentation broth by contacting the fermentation broth with a hydrophobic organic extraction solvent, and extracting the statin compound from the fermentation broth into the hydrophobic solvent;
- (c) separating the hydrophobic solvent from the fermentation broth;
- (d) concentrating a solution of the hydrophobic solvent containing the extracted statin compound;
- (e) washing the concentrated solution of the hydrophobic solvent with an aqueous solution containing base;
- and
- (f) purifying the extracted statin compound by crystallization.
Dependencies are inferred from the claim text.