Apparatus for screening compound libraries
AssigneesOle Hindsgaul, David C. Schriemer
InventorsOle Hindsgaul, David C. Schriemer
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Abstract
Disclosed are apparatus for screening compound libraries using frontal chromatography in combination with mass spectrometry to identify and rank those members of the library that bind to a target receptor. The apparatus of this invention also permit a compound library to be rapidly screened to determine if any member of the library has a higher affinity for the target receptor relative to a pre-selected indicator compound.
Claims18
An apparatus for screening a plurality of compound libraries to determine the relative or absolute affinity of a plurality of putative ligands in each library to a target receptor, which apparatus comprises:
- (a) a plurality of columns each comprising a target receptor optionally attached to a solid phase support and having a inflow end and an outflow end, wherein each of said columns is capable of independently having a compound library comprising a plurality of putative ligands continuously applied thereto under frontal chromatography conditions whereby the target receptor is continuously contacted with the compound library to produce an effluent from the outflow end of the column;
- (b) a plurality of first reservoir connected to the inflow end of one of the columns for continuously applying the compound library to the columns;
- (c) a mass spectrometer connected to the outflow end of each of said columns for intermittently analyzing the effluent from each of the column.
Dependencies are inferred from the claim text.