Methods for producing polypeptides in aspergillus mutant cells
AssigneeNovozymes A/S
InventorsBjorn Eggert Christensen, Henrik Mollgaard, Svend Kaasgaard, Jan Lehmbeck
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Abstract
A method is provided for producing a polypeptide of interest by (a) cultivating a mutant of a parent Aspergillus cell, wherein (i) the mutant comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of at least one toxin, and (ii) the mutant produces less of the toxin than the parent Aspergillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the culture medium. Also, mutants of Aspergillus cells are provided, as well as methods for obtaining the mutant cells.
Claims16
A method for producing a polypeptide, said method comprising:
- (a) cultivating a mutant of a parent Aspergillus cell, wherein (i) the mutant comprises a nucleic acid sequence encoding said polypeptide, and (ii) the mutant produces less of at least one toxin selected from the group consisting of emodin, kojic acid, malformin, 3-nitropropionic acid, ochratoxins, and secalonic acids than the parent Aspergillus cell when cultured under the same conditions;
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- (b) isolating the polypeptide from the culture medium.
Dependencies are inferred from the claim text.