Enzymatic conversion in a solvent mixture containing water and flourinated, non-chlorinated solvent
AssigneeAdvanced Phytonic Limited
InventorMazin Nicola
Life of this patent
Estimating the term…
Abstract
A first compound is converted to a second compound enzymatically in a solvent mixture containing water and a fluorinated, non-chlorinated alkane, alkene, or alkyne having up to 4 carbon atoms. Lactams, for example 6-aminopenicillanic acid (6-APA), may be prepared by enzymatic conversion of a first compound, for example penicillin-G, in a solvent mixture comprising water and a non-aqueous organic solvent, for example 1,1,1, 2-tetrafluoroethane. The 6-APA can be caused to precipitate, isolated by filtration and optionally derivatized to produce a desired compound. A by-product of the enzymatic conversion, for example phenylacetic acid, can be isolated by solvent extraction, suitably using a solvent which also comprises 1, 1, 1, 2-tetrafluoroethane.
Claims23
A method of preparing a second compound by enzymatic catalytic conversion of a first compound, the method comprising contacting said first compound and an enzyme in a first solvent mixture comprising water and a first non-aqueous solvent, wherein said first non-aqueous solvent is a fluorinated, non-chlorinated alkane, alkene or alkyne having up to four carbon atoms, and allowing said enzyme to convert said first compound to said second compound.
Dependencies are inferred from the claim text.