Polymeric encapsulation system promoting angiogenesis

A method of forming a polymeric drug delivery vehicle comprised of a polyanionic/polycationic polymer wherein said polycation contacts said polyanion through a dialysis process, comprising the steps of:

• a) introducing a polyanion solution into a dialysis cassette;
• wherein the polyanion solution comprises a polyanion selected from the group consisting of kappa carrageenan, low-esterified pectin, polyglutamic acid, carboxymethylcellulose, chondroitin sulfate-6, chondroitin sulfate-4, collagen, high viscosity sodium alginate, and cellulose sulfate and mixtures thereof;
• b) immersing said cassette in a stirred reactor containing a polycation solution;
• wherein the polycation solution comprises a polycation selected from the group consisting of polyvinylamine, spermine hydrochloride, protamine sulfate, polyethyleimine, polyethyleimine-exthoxylated, polyethyleimine-epichlorhydrin modified, quartenized polyamide, polydiallyldimethyl ammonium chloride-co-acrylamide, low molecular-weight chitosan, poly(methylene-co-guanidine) and calcium chloride and mixtures thereof and,
• c) allowing dialysis to proceed for a period of time to allow formation of a polyanionic/polycationic polymer shell comprising said drug delivery vehicle;
• wherein a protein is incorporated into the polymer drug delivery vehicle by adding said protein to said polyanion solution.

A method of coating prefabricated materials with a polyanionic/polycationic polymer containing an angiogenic stimulating factor, comprising the steps of:

• a) applying a coat of a polyanion solution to said material, wherein said solution contains an angiogenic stimulating factor;
• wherein said angiogenic stimulating factor is incorporated into said polymer
• b) allowing said polyanion coat to dry;
• c) applying a coat of a polycation solution to said material;
• d) allowing said polycation coat to dry;
• and,
• e) sequentially repeating steps a through d.