Herpesvirus saimiri as viral vector
AssigneeUniversity of Leeds
InventorsDavid Mark Meredith, Alexander Fred Markham
Life of this patent
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Abstract
The invention relates to herpesvirus saimiri viruses that are genetically modified by mutations and/or deleting specific essential and non-essential genes. The essential genes are required in replication of viral genes and are needed for viral proliferation. The non-essential genes can represent sites for the insertion of heterologous genetic material.
Claims32
An isolated herpesvirus saimiri having at least one mutation in at least one of genes ORF 50 and ORF 57 and wherein the at least one mutation prevents viral replication in a human cell.
An isolated herpesvirus saimiri having at least one mutation in at least one of genes ORF 4, ORF 14, ORF 15, ORF 16 and ORF 51.
An isolated herpesvirus saimiri having at least one mutation in at least one of genes ORF 4, ORF 14, ORF 15, ORF 16 and ORF 51 and additionally having therein at least one pre-selected heterologous DNA fragment in a deletion site, which site represents a site for partial or whole deletion of at least one gene encoding a non-essential protein and which virus is additionally mutated or deleted for a gene encoding ORF 50 or ORF 57.
An isolated herpesvirus saimiri having therein at least one pre-selected heterologous DNA fragment in the junction of a single coding region and a non-coding region;
- and further wherein the virus comprises a reduced number of repetitive non-coding sequences at one or both ends of the single coding region;
- and the virus also comprises at least one mutation in at least one gene encoding ORF 50 or ORF 57.
An isolated herpesvirus saimiri having at least one mutation in one of genes ORF 50 and ORF 51 wherein said mutation prevents viral replication in a human cell and a mutation in a gene encoding a non-essential protein.
A method for delivering selected heterologous DNA to a target cell by infecting said cell with a recombinant herpesvirus saimiri virus having both at least one mutation in at least one of genes ORF 50 and ORF 57 and a pre-selected heterologous DNA.
A method for delivering selected heterologous DNA to a target cell by infecting said cell with a recombinant herpesvirus saimiri virus having both at least one mutation in at least one of genes ORF 4, ORF 14, ORF 15, ORF 16 and ORF 51 and a pre-selected heterologous DNA.
Dependencies are inferred from the claim text.