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US 6,379,933 B1Granted
Method of transferring at least two saccharide units with a polyglycosyltransferase
AssigneeNeose Technologies, Inc.
InventorsKarl F. Johnson, Stephen Roth, Stephanie L. Buczala
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Abstract
The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.
Claims18
A method for synthesizing a saccharide composition, comprising:
- (a) contacting a first saccharide donor with a first acceptor moiety in the presence of a polyglycosyltransferase that catalyzes the linkage of a first saccharide to the first acceptor moiety to form an intermediate saccharide composition;
- and
- (b) contacting a second saccharide donor with the intermediate saccharide composition formed in step (a) or a derivative thereof in the presence of the polyglycosyltransferase that also catalyzes the linkage of a second saccharide to a second acceptor moiety in the intermediate saccharide composition or derivative,
- wherein the relative rate of a first glycosyltransferase activity of the polyglycosyltransferase is 0.8 to 1.5 times the rate of a second glycosyltransferase activity of the polyglycosyltransferase, and wherein the first and second glycosyltransferase activities are each within the range of from 1 to 250 turnovers per second.
1 more element
Dependencies are inferred from the claim text.