Spectroscopic diagnostics for bacteria in biologic sample
AssigneesGeorgia Tech Research Corporation, Boston Medical Center Corporation
InventorsMostafa A. El-Sayed, Ivan H. El-Sayed
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Abstract
A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.
Claims26
A method for detecting bacteria in a biologic fluid sample comprising the steps of:
- a. obtaining absorption spectra directly from an uncultured biologic fluid sample;
- b. subtracting absorption spectra of a reference sample from the absorption spectra of the biologic fluid sample to form a differential spectra;
- c. analyzing the differential spectra to detect the presence of bacteria in the sample.
A method for decreasing the time for diagnosing a bacterial strain infection comprising the steps of:
- a. obtaining the absorption spectra of an uncultured biologic fluid sample;
- b. subtracting the absorption spectra of a previously-obtained reference sample from the absorption spectra of the uncultured biologic fluid sample to obtain a differential spectra;
- c. analyzing the differential spectra to detect the presence of bacteria in the sample.
A method for the in vivo detection of bacteria comprising the steps of:
- a. obtaining the absorption spectra of an uncultured biologic fluid sample directly from a body;
- b. subtracting absorption spectra of a reference sample from the absorption spectra of the biologic fluid sample to form a differential spectra;
- c. analyzing the differential spectra to detect the presence of bacteria in the sample.
A method for diagnosing otitis media comprising the steps of:
- a. obtaining the absorption spectra directly from an uncultured sample of middle ear aspirate;
- b. subtracting the spectrum of a previously-obtained reference sample from the spectra of the middle ear aspirate;
- c. analyzing the differential spectra to detect the presence of bacteria in the sample.
Dependencies are inferred from the claim text.