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US 6,379,883 B2Granted
Microfabricated aperture-based sensor
AssigneeI-Stat Corporation
InventorsGraham Davis, Chao Lin, Imants R. Lauks, Raymond J. Pierce
Life of this patent
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Abstract
A chemical sensor includes an enzyme layer, a diffusion layer and an analyte barrier layer positioned over the diffusion layer. Apertures are formed by microfabrication in the analyte barrier layer to allow controlled analyte flux to the diffusion layer.
Claims10
A method of measuring the concentration of an analyte in a sample that contains a co-reactant, the method comprising:
- (a) providing a sample suspected of containing an analyte of interest, the sample comprising a co-reactant;
- (b) contacting the sample with a layer comprising a microfabricated aperture and a layer comprising a catalyst, wherein the co-reactant contacts the catalyst by diffusing substantially freely through the layer comprising the microfabricated aperture, and the analyte contacts the catalyst substantially through the aperture;
- (c) obtaining at least one detectable product from a reaction between the analyte, the co-reactant and the catalyst;
- and
- (d) detecting the product with a sensor thereby producing an output that depends upon the concentration of the analyte in the sample.
2 more elements
Dependencies are inferred from the claim text.