Antigenic preparations
AssigneeBoehringer Indgelheim Vetmedica GmbH
InventorsDieter Farnow, Joachim Karle, Igor D. Poliakov, Ludmilla G. Ivanova
Life of this patent
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Abstract
The present invention relates to antigenic preparations comprising polysaccharides and/or glycopeptides preparable from keratinophilic fungi as well as yeasts, processes for the preparation of these antigenic preparations, their use as pharmaceutical substances as well as their use as vaccines, including but not limited to, the prophylaxis and treatment of allergy, as well as for modulating the immune response.
Claims30
A method for prophylaxis, treatment, or prophylaxis and treatment of an immunological disease in a mammal in need thereof, comprising administering to said mammal an effective amount of an antigenic material comprising one or more substances selected from the group consisting of polysaccharide and glycopeptide, wherein said antigenic material comprises one or more fractions independently selected from the group consisting of:
- (a) ASMP fractions, wherein each of said ASMP fractions is obtained by a process comprising:
- (i) contacting cells, of one or more microorganisms selected from said group consisting of keratinophilic fungi and keratinophilic yeast, or parts thereof, with an aqueous alkaline solution, thereby obtaining a preparation;
- (ii) separating said preparation into solid and liquid phases, thereby obtaining a first supernatant;
- (iii) contacting said first supernatant with mineral or organic acid and allowing solid and liquid phases to separate, thereby obtaining a second supernatant;
- and
- (iv) precipitating said ASMP fraction out of said second supernatant;
- (b) ANMP fractions, wherein each of said ANMP fractions is obtained by a process comprising:
- (i) contacting cells, of one or more microorganisms selected from said group consisting of keratinophilic fungi and keratinophilic yeast, or parts thereof, with an aqueous alkaline solution, thereby obtaining a preparation;
- (ii) separating said preparation into solid and liquid phases, thereby obtaining a first solid phase;
- (iii) contacting said first solid phase with mineral or organic acid, thereby obtaining a second solid phase comprising said ANMP fraction;
- (c) AEMP1 fractions, wherein each of said AEMP1 fractions is obtained by a process comprising:
- (i) cultivating in liquid medium cells of one or more microorganisms selected from said group consisting of keratinophilic fungi and keratinophilic yeast, thereby obtaining a culture;
- (ii) separating said culture into solid and liquid phases, thereby obtaining a supernatant;
- and
- (iii) precipitating said AEMP1 fraction out of said supernatant;
- and
- (d) AEMP2 fractions, wherein each of said AEMP2 fractions is obtained by a process comprising:
- (i) cultivating in liquid medium cells of one or more microorganisms selected from said group consisting of keratinophilic fungi and keratinophilic yeast, thereby obtaining a culture;
- (ii) separating said culture into solid and liquid phases, thereby obtaining a supernatant;
- (iii) lyophilizing said supernatant, thereby obtaining a first dry matter;
- (iv) dissolving said first dry matter in an aqueous solution, thereby obtaining a first aqueous solution;
- (v) precipitating said first aqueous solution with 1-5 volumes of an alcohol, thereby obtaining a precipitate;
- (vi) dissolving said precipitate in an aqueous solution, thereby obtaining a second aqueous solution;
- and
- (vii) lyophilizing said second aqueous solution, thereby obtaining a second dry matter comprising said AEMP2 fraction.
The method of 6, wherein said alcohol is a lower alcohol.
A method for prophylaxis, treatment, or prophylaxis and treatment of an immunological disease in a mammal in need thereof, comprising administering to said mammal an effective amount of an antigenic material soluble in aqueous solutions (ASMP) comprising at least one material selected from a group consisting of polysaccharide and glycopeptide, wherein said immunological disease is summer eczema and said ASMP is obtained by a process comprising:
- (i) contacting Trichophyton mentagrophytes DSM 7279, or parts thereof, with an aqueous alkaline solution, thereby obtaining a preparation;
- (ii) separating said preparation into solid and liquid phases, thereby obtaining a first supernatant;
- (iii) contacting said first supernatant with an organic or mineral acid and allowing solid and liquid phases to separate, thereby obtaining a second supernatant;
- and
- (iv) precipitating said ASMP out of said second supernatant.
Dependencies are inferred from the claim text.