Submucosa extracts
AssigneePurdue Research Foundation
InventorsStephen F. Badylak, Andrew O. Brightman, Jason P. Hodde, Timothy B. McPherson, Sherry L. Voytik-Harbin
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Abstract
A method for preparing a bioactive extract from warm-blooded vertebrate submucosal tissue is described. The submucosal tissue is extracted using an aqueous solution of extraction excipients, and the extracted bioactive components are then separated from the extraction excipients to provide an isolated extract enriched in bioactive components. The isolated extract is useful, inter alia, as additive for cell growth media to promote cell growth and proliferation in vitro.
Claims27
A method for preparing a bioactive composition useful for promoting cell growth comprising the steps of extracting submucosal tissue with an aqueous solution of extraction excipients to form an aqueous solution of extracted bioactive components and extraction excipients, and separating the extracted bioactive components from at least a portion of the extraction excipients to form the bioactive composition.
An isolated extract of vertebrate submucosa comprising a solution of bioactive components extracted from native vertebrate submucosa and substantially free of extraction excipients.
An isolated extract of intestinal vertebrate submucosa comprising a solution of bioactive components extracted from native intestinal vertebrate submucosa and substantially free of extraction excipients.
A method of manufacturing an extract enriched for a bioactive component of submucosal tissue of a warm blooded vertebrate, said method comprising the steps of extracting the submucosal tissue with an aqueous solution of extraction excipients to form an aqueous solution of extracted bioactive components and extraction excipients, and separating the extracted bioactive components from the extraction excipients to form a bioactive extract.
Dependencies are inferred from the claim text.