Plants with modified stamen cells
AssigneeAventis CropScience GmbH
InventorsCelestina Mariani, Jan Leemans, Willy De Greef, Marc De Beuckeleer
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Abstract
A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a protein or polypeptide which disrupts the metabolism, functioning and/or development of stamen cells of the plant, said foreign DNA under the control of a stamen-specific promoter. The foreign DNA sequence also optionally encodes a marker.
Claims67
A cell of a plant, the nuclear genome of said cell being transformed with a foreign DNA sequence;
- said foreign DNA sequence comprising:
- a) a male-sterility DNA encoding a first protein or polypeptide which, when produced or overproduced in a stamen cell of said plant, disturbs significantly one or more activities of said stamen cell selected from the group consisting of the metabolism, functioning and development of said stamen cell;
- and
- b) a first promoter capable of directing expression of said male-sterility DNA selectively in stamen cells of said plant;
- said male-sterility DNA being in the same transcriptional unit as, and under the control of, said first promoter;
- whereby said stamen cells of said plant are killed or disabled by expression of said male sterility DNA selectively in said stamen cells of said plant, so as to render said plant incapable of producing fertile male gametes.
A plant consisting essentially of cells, the nuclear genome of said cells being transformed with a foreign DNA sequence;
- said foreign DNA sequence comprising:
- a) a male-sterility DNA encoding a first protein or polypeptide which, when produced or overproduced in a stamen cell of said plant, disturbs significantly one or more activities of said stamen cell selected from the group consisting of the metabolism, functioning and development of said stamen cells;
- and
- b) a first promoter capable of directing expression of said male-sterility DNA selectively in stamen cells of said plant;
- said male-sterility DNA being in the same transcriptional unit as, and under the control of, said first promoter;
- whereby said stamen cells of said plant are killed or disabled by expression of said male-sterility DNA selectively in said stamen cells of said plant;
- so as to render said plant incapable of producing fertile male gametes.
A plant consisting essentially of cells, the nuclear genome of said cells being transformed with a foreign DNA sequence;
- said foreign DNA sequence comprising:
- a) a male-sterility DNA encoding barnase;
- b) a first promoter capable of directing expression of said male-sterility DNA selectively in stamen cells of said plant;
- said male-sterility DNA being in the same transcriptional unit as, and under the control of, said first promoter;
- whereby said stamen cells of said plant are killed or disabled by expression of said male-sterility DNA selectively in said stamen cells of said plant, so as to render said plant incapable of producing fertile male gametes.
Dependencies are inferred from the claim text.