Hemoglobin genes and their use
AssigneePioneer Hi-Bred International, Inc.
InventorsMitchell C. Tarczynski, Bo Shen
Life of this patent
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Abstract
The invention relates to the genetic manipulation of plants, particularly to the expression of hemoglobin genes in transformed plants. Nucleotide sequences for the hemoglobin genes and methods for their use are provided. The sequences find use in enhancing seed germination, seedling growth, and overall growth and metabolism of the plant.
Claims33
An isolated nucleotide sequence selected from the group consisting of:
- A) a nucleotide sequence encoding the amino acid sequence of SEQ ID NOS:
- 2 or 4;
- B) a nucleotide sequence set forth in SEQ ID NOS:
- 1 or 3;
- C) a polynucleotide encoding a hemoglobin polypeptide, said polynucleotide having at least 70 percent sequence identity to SEQ ID NOS:
- 1 or 3, wherein the percent sequence identity is based on the entire sequence and is determined by GAP analysis using default parameters;
- and
- D) a polynucleotide complementary to the entire length of a polynucleotide of (A) through, (C).
A method for enhancing seed germination and seedling growth, said method comprising transforming a plant with at least one nucleotide sequence encoding a hemoglobin protein said nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- A) a nucleotide sequence encoding the amino acid sequence of SEQ ID NOS:
- 2 or 4;
- B) a nucleotide sequence set forth in SEQ ID NOS:
- 1 or 3;
- C) a polynucleotide having at least 70 percent sequence identity to SEQ ID NOS:
- 1 or 3, wherein the percent sequence identity is based on the entire sequence and is determined by GAP analysis using default parameters;
- and
- D) a polynucleotide complementary to the entire length of a polynucleotide of (A) through, (C).
A method for manipulating oxygen concentration in a plant cell, said method comprising transforming said plant cell with at least one nucleotide sequence encoding a hemoglobin protein said nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- A) a nucleotide sequence encoding the amino acid sequence of SEQ ID NOS:
- 2 or 4;
- B) a nucleotide sequence set forth in SEQ ID NOS:
- 1 or 3;
- C) a polynucleotide having at least 70 percent sequence identity to SEQ ID NOS:
- 1 or 3, wherein the percent sequence identity is based on the entire sequence and is determined by GAP analysis using default parameters;
- and
- D) a polynucleotide complementary to the entire length of a polynucleotide of (A) through (C).
A transformed plant cell having stably incorporated into its genome at least one nucleotide sequence encoding a hemoglobin protein said nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NOS:
- 2 or 4;
- b) a nucleotide sequence set forth in SEQ ID NOS:
- 1 or 3;
- c) a polynucleotide having at least 70 percent sequence identity to SEQ ID NOS:
- 1 or 3, wherein the percent sequence identity is based on the entire sequence and is determined by GAP analysis using default parameters;
- and
- d) a polynucleotide complementary to the entire length of a polynucleotide of (A) through (C);
- and wherein said transformed plant cell has been transformed with said nucleotide sequence.
A transformed plant having stably incorporated into its genome at least one nucleotide sequence encoding a hemoglobin protein said nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NOS:
- 2 or 4;
- b) a nucleotide sequence set forth in SEQ ID NOS:
- 1 or 3;
- c) a polynucleotide having at least 70 percent sequence identity to SEQ ID NOS:
- 1 or 3, wherein the percent sequence identity is based on the entire sequence and is determined by GAP analysis using default parameters;
- and
- d) a polynucleotide complementary to the entire length of a polynucleotide of (A) through (C);
- and wherein said transformed plant has been transformed with said nucleotide sequence.
A method for modulating hemoglobin levels in a plant cell, said method comprising transforming said plant cell with at least one nucleotide sequence encoding a hemoglobin protein, said nucleotide sequence operably linked to a promoter in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- A) a nucleotide sequence encoding the amino acid sequence of SEQ ID NOS:
- 2 or 4;
- B) a nucleotide sequence set forth in SEQ ID NOS:
- 1 or 3;
- C) a polynucleotide having at least 70 percent sequence identity to SEQ ID NOS:
- 1 and 3, wherein the percent sequence identity is based on the entire sequence and is determined by GAP analysis using default parameters;
- and
- D) a polynucleotide complementary to the entire length of a polynucleotide of (A) through (C).
An isolated nucleotide sequence having at least 50 nucleotides in length which hybridizes under high stringency conditions, including a wash in 0.1×SSC to 60 to 65° C., to a polynucleotide having the sequence set forth in SEQ ID NOS:
- 1 or 3.
Dependencies are inferred from the claim text.