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US 6,372,721 B1Granted
Method for inducing DNA synthesis in neurons
AssigneeSpinal Cord Society
InventorsToomas Neuman, Kikuo Suda, Howard O. Nornes
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AssigneeSpinal Cord Society
InventorsToomas Neuman, Kikuo Suda, Howard O. Nornes
Estimating the term…
A method is provided for inducing DNA synthesis in differentiated neurons. According to certain embodiments of the invention, a method for inducing DNA synthesis in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a differentiated neuron, and transfecting a differentiated neuron with the vector. According to certain embodiments of the invention, a method for integrating DNA encoding a desired protein in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a neuron, obtaining DNA encoding a desired protein, and cotransfecting a differentiated neuron with the vector and the DNA encoding the desired protein such that the DNA encoding the desired protein is integrated in the differentiated neuron and the desired protein is produced.
A method for inducing nucleic acid synthesis in a differentiated neuron in vivo comprising:
A method for integrating DNA encoding a desired protein in a differentiated neuron in vivo comprising:
An improved method of inducing neurons to express DNA encoding a desired protein of the type wherein the DNA encoding the desired protein is introduced into the neuron, the improvement comprising:
An improved method of inducing neurons to express DNA encoding a desired protein of the type wherein the DNA encoding the desired protein is introduced into the neuron, the improvement comprising:
A method for integrating DNA encoding a desired protein into a differentiated neuron, the method comprising co-transfecting a differentiated neuron with DNA encoding a desired protein and DNA encoding either (a) an E2F regulator, (b) an E1A regulator, or (c) both an E2F regulator and an E1A regulator wherein the co-transfection step is performed in vitro.
A method for integrating DNA encoding a desired protein into a differentiated neuron, the method comprising co-transfecting a differentiated neuron with DNA encoding a desired protein and DNA encoding either (a) an E2F regulator, (b) an E1A regulator, or (c) both an E2F regulator and an E1A regulator wherein the co-transfection step is performed in vivo in an animal, the animal being either human or non-human.
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