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US 6,372,506 B1Granted
Apparatus and method for verifying drop delay in a flow cytometer
AssigneeBecton, Dickinson and Company
InventorPierce Owen Norton
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AssigneeBecton, Dickinson and Company
InventorPierce Owen Norton
Estimating the term…
An apparatus and method for verifying the drop delay in a flow cytometer, which is indicative of the time that elapses from a moment at which a particle of interest is detected at an interrogation point in the flow of cytometer to the moment at which a sorting condition, such as a charging potential, is applied in response to the detection to a droplet forming at a droplet forming location in the flow cytometer. The apparatus and method analyzes the content of the droplets formed by the flow cytometer, and based on the analysis, provides an indication as to whether the drop delay time at which the flow cytometer is operating is correct. Specifically, the charging potential should be applied to the droplet containing the detected particle of interest. The content of the droplets can be analyzed at the droplet formation point, or at a location downstream of the droplet formation point, to detect the presence or absence of a particle of interest that was detected at the interrogation point in the flow cytometer. The drop delay time at which the droplets being formed by the flow cytometer are charged can then be set based on the results of the analysis of the content of the droplets, to ensure that droplets containing particles of interest are charged and thus sorted by the flow cytometer, while droplets not including particles of interest remain uncharged and are not sorted.
An apparatus, for use in a flow cytometer, for verifying accuracy of a drop delay setting representing a period of time elapsing between a moment at which any of a plurality of particles in a fluid flowing through said flow cytometer is detected at a reference location and a moment at which a sorting condition is applied in response to said detection of a droplet of said fluid forming at a droplet forming location, said apparatus comprising:
An apparatus, for use in a flow cytometer, for verifying accuracy of a drop delay setting representing a period of time elapsing between a moment at which any of a plurality of particles in a fluid flowing through said flow cytometer is detected at a reference location and a moment at which a sorting condition is applied in response to said detection of a droplet of said fluid forming at a droplet forming location, said apparatus comprising:
A method for verifying accuracy of a drop delay setting in a flow cytometer, said drop delay representing a period of time elapsing between a moment at which any of a plurality of particles in a fluid flowing through said flow cytometer is detected at a reference location to a moment at which a sorting condition is applied in response to said detection of a droplet of said fluid forming at a droplet forming location, said method comprising the steps of:
A method for verifying accuracy of a drop delay setting in a flow cytometer, said drop delay representing a period of time elapsing between a moment at which any of a plurality of particles in a fluid flowing through said flow cytometer is detected at a reference location to a moment at which a sorting condition is applied in response to said detection of a droplet of said fluid forming at a droplet forming location, said method comprising the steps of:
Dependencies are inferred from the claim text.