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AssigneeVivascience AG
InventorVincenzo Vassarotti
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A device for removing or replacing solvent in a liquid sample containing macromolecules includes a concentration chamber having an aperture adjacent to the upper end of the concentration chamber which is arranged to receive and tightly fix the outlet of a detachable auxiliary reservoir. In methods employing the device, the concentration chamber is filled with part of a liquid sample to a predetermined level, and a detachable auxiliary upper reservoir containing the rest of the liquid sample is tightly fixed to the aperture adjacent to the upper end of the concentration chamber to successively deliver additional liquid sample as the concentration is progressing, or a detachable auxiliary upper reservoir containing a solvent or buffer solution is tightly fixed to the aperture adjacent to the upper end of the concentration chamber to successively deliver solvent or buffer solution as a replacement for the liquid absorbed by an absorption material.
In a device for removing or replacing solvent in a liquid sample containing macromolecules comprising a concentration chamber (3) having at least one rigid wall and one opposite wall formed from a membrane (7) permeable to said solvent, an aperture adjacent an upper part of said concentration chamber for introduction and removal of liquid, a pocket (11) in its lower portion for retaining a fixed volume of a concentrated sample, and an absorption container (1) provided with absorbent material (10) arranged closely adjacent said membrane wall of said concentration chamber and capable of absorbing said solvent, the improvement comprising said aperture adjacent the upper end of said concentration chamber arranged to receive an outlet of a detachable auxiliary upper reservoir (5), and means for providing gas-tight or liquid-tight seal between said auxiliary reservoir and the concentration chamber when said auxiliary reservoir is received in said aperture in the concentration chamber.
In a device for removing or replacing solvent in a liquid sample containing macromolecules comprising a concentration chamber (3) having at least one rigid wall and one opposite wall formed of a membrane (7) permeable to said solvent, an aperture adjacent an upper part of said concentration chamber for introduction and removal of liquid, a pocket (11) in its lower portion for retaining a fixed volume of a concentrated sample, and an absorption container (1) provided with absorbent material (10) arranged closely adjacent said membrane wall of said concentration chamber and capable of absorbing said solvent, the improvement comprising a detachable auxiliary upper reservoir (5) arranged fixed to said aperture adjacent the upper end of said concentration chamber, and means for providing a gas-tight or liquid-tight seal between said auxiliary reservoir and the concentration chamber (3) when said auxiliary reservoir (5) is received in said aperture in the concentration chamber (3).
In a method for concentrating a liquid sample containing macromolecules and a solvent by means of a device comprising a concentration chamber (3) having at least one rigid wall and one opposite wall formed of a membrane (7) permeable to said solvent, an aperture adjacent the upper part of said concentration chamber for introduction and removal of liquid, a pocket (11) in its lower portion for retaining a fixed volume of a concentrated sample, and an absorption container (1) provided with absorbent material (10) arranged closely adjacent said membrane wall of said concentration chamber and capable of absorbing said solvent, the improvement comprising the following steps:
In a method for washing macromolecules in a liquid sample containing macromolecules and a solvent by means of a device comprising a concentration chamber (3) having at least one rigid wall and one opposite wall formed of a membrane (7) permeable to said solvent, an aperture adjacent the upper part of said concentration chamber for introduction and removal of liquid, a pocket (11) in its lower portion for retaining a fixed volume of a concentrated sample, and an absorption container (1) provided with absorbent material (10) arranged closely adjacent said membrane wall of said concentration chamber and capable of absorbing said solvent, the improvement comprising the following steps:
Dependencies are inferred from the claim text.