Diluent for cryogenic storage of bovine spermatozoa

A process for preparing a diluent for cryogenic storage of bovine spermatozoa, comprising a phospholipid, a liposoluble vitamin accompanied by an antioxidant peptide or amino acid with antioxidant properties, and a polyol, including the following steps:

• a) preparing a dispersion of a phospholipid in the form of particles in a polyol;
• b) stirring the dispersion from step a) to micronize said phospholipid particles;
• c) leaving the dispersion obtained in step b) to stand for a period of at least about 12 hours to stabilize the emulsion obtained, yielding a preparation referred to as the preparation from step c),
• d) preparing an aqueous phase including an amino acid with antioxidant properties or an antioxidant peptide, and a liposoluble vitamin, yielding a phase referred to as the aqueous phase from step d),
• e) combining said preparation from step c) and said aqueous phase from step d), yielding a preparation referred to as the preparation from step e), and
• f) sterilizing said preparation from step e).