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US 6,365,418 B1Granted
Arrays of protein-capture agents and methods of use thereof
AssigneeZyomyx, Inc.
InventorsPeter Wagner, Steffen Nock, Dana Ault-Riche, Christian Itin
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Abstract
Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.
Claims6
An array device comprising
- a substrate having a surface;
- and,
- one or more protein immobilization regions on said surface, said protein immobilization regions each comprising;
- (i) an ordered hydrophobic monolayer formed of alkyl chains having proximal ends which are chemisorbed or physisorbed to said surface within said immobilization regions, and opposite hydrophobic distal ends,
- (ii) a hydrophilic monolayer attached to said ordered hydrophobic monolayer, said hydrophilic monolayer comprising hydrophilic chains, each hydrophilic chain having a proximal end by which said hydrophilic chain is linked to an alkyl chain distal end, and an opposite hydrophilic distal end, and,
- (iii) a plurality of functional groups attached to said hydrophilic/hydrophobic monolayers for covalently attaching protein-capture agents to said hydrophilic/hydrophobic monolayers within said immobilization regions;
- wherein said hydrophobic monolayer and hydrophilic monolayers together are effective to resist non-specific protein binding.
4 more elements
Dependencies are inferred from the claim text.