Recombination of insertion modified nucleic acids

A method of recombining a first and a second target nucleic acid, the method comprising:

• providing a first and a second target nucleic acid, wherein at least one of the first and second target nucleic acids comprises a plurality of homologous or non-homologous insertion nucleic acid sequences, wherein the insertion nucleic acid sequences comprise at least one intein;
• and,
• recombining the first and second target nucleic acids, thereby providing a recombinant nucleic acid,
• wherein the recombinant nucleic acid encodes a protein subsequence, and, wherein the protein subsequence and the second protein subsequence are spliced in vitro to produce an active protein.

A method of recombining a first and a second target nucleic acid, the method comprising:

• providing one or more parental nucleic acids, wherein at least one of the parental nucleic acids is recombined with a plurality of insertion nucleic acid sequences, thereby providing a first or a second target nucleic acid, wherein at least one of the first and second target nucleic acids comprises the plurality of homologous or non-homologous insertion nucleic acid sequences;
• and,
• recombining the first and second target nucleic acids, thereby providing a recombinant nucleic acid,
• wherein the parental nucleic acid corresponds to one or more of:
• a gene or cDNA encoding EPO, a gene or cDNA encoding an insulin protein, a gene or cDNA encoding a peptide hormone, a gene or cDNA encoding a cytokine, a gene or cDNA encoding an epidermal growth factor, a gene or cDNA encoding a fibroblast growth factor, a gene or cDNA encoding a hepatocyte growth factor, a gene or cDNA encoding insulin-like growth factor, a gene or cDNA encoding an interferon, a gene or cDNA encoding an interleukin, a gene or cDNA encoding a keratinocyte growth factor, a gene or cDNA encoding a leukemia inhibitory factor, a gene or cDNA encoding oncostatin M, a gene or cDNA encoding PD-ECSF, a gene or cDNA encoding PDGF, a gene or cDNA encoding pleiotropin, a gene or cDNA encoding SCF, a gene or cDNA encoding c-kit ligand, a gene or cDNA encoding VEGF, a gene or cDNA encoding G-CSF, a gene or cDNA encoding an oncogene, a gene or cDNA encoding a tumor suppressor, a gene or cDNA encoding a steroid hormone receptor, a gene or cDNA encoding a plant hormone, a gene or cDNA encoding a disease resistance gene, a gene or cDNA encoding an herbicide resistance gene, a gene or cDNA encoding a bacterial gene, a gene or cDNA encoding a monooxygenase, a gene or cDNA encoding a protease, a gene or cDNA encoding a nuclease, a gene or cDNA encoding a lipase, a gene or cDNA encoding a C-X-C chemokine, a gene or cDNA encoding a C—C chemokine, a gene or cDNA encoding an antibody V gene, a gene or cDNA encoding a cystein knot protein, a gene or cDNA encoding TGFβ, a gene or cDNA encoding NGF, a gene or cDNA encoding PDGFβ a gene or cDNA encoding a TNKor family member, a gene or cDNA encoding CNTF, a gene or cDNA encoding 4F, a gene or cDNA encoding an RNase, a gene or cDNA encoding an antibody, a gene or cDNA encoding peptide ligand, a gene or cDNA encoding a tumor necrosis factor and a gene or cDNA encoding an angiogenisis inhibitor.

The method of recombining a first and a second target nucleic acid, the method comprising:

• providing a first parental nucleic acid sequence and a second parental nucleic acid sequence, wherein the first and second parental nucleic acid sequences are homologous or non-homologous;
• inserting a plurality of insertion nucleic acid sequences into one or more of the first and second parental nucleic acid sequences, thereby providing first and second target nucleic acids, wherein the first and second parental nucleic acid sequences hybridize under stringent conditions, and the first and second target nucleic acids do not hybridize under stringent conditions;
• and,
• recombining the first and second target nucleic acids, wherein at least one of the first and second target nucleic acids comprises the plurality of homologous or non-homologous insertion nucleic acid sequences, thereby providing a recombinant nucleic acid.

The method of recombining a first and a second target nucleic acid, the method comprising:

• providing a first parental nucleic acid sequence and a second parental nucleic acid sequence, wherein the first and second parental nucleic acid sequences are homologous or non-homologous;
• inserting a plurality of insertion nucleic acid sequences into one or more of the first and second parental nucleic acid sequences, thereby providing the first and the second target nucleic acids, wherein the first and second parental nucleic acid sequences do not hybridize under stringent conditions, and wherein the first and second target nucleic acids hybridize under stringent conditions;
• and,
• recombining the first and second target nucleic acids, wherein at least one of the first and second target nucleic acids comprises the plurality of homologous or non-homologous insertion nucleic acid sequences, thereby providing a recombinant nucleic acid.

The method of recombining a first and a second target nucleic acid, the method comprising:

• providing a first parental nucleic acid sequence and a second parental nucleic acid sequence, wherein the first and second parental nucleic acid sequences are homologous or non-homologous;
• inserting a plurality of insertion nucleic acid sequences into one or more of the first and second parental nucleic acid sequences, thereby providing the first and the second target nucleic acids, wherein the first and second target nucleic acids hybridize under stringent conditions, and wherein the first target nucleic acid does not hybridize under stringent conditions to the second parental nucleic acid, or wherein the second target nucleic acid does not hybridize under stringent conditions to the first parental nucleic acid sequence;
• and,
• recombining the first and second target nucleic acids, wherein at least one of the first and second target nucleic acids comprises the plurality of homologous or non-homologous insertion nucleic acid sequences, thereby providing a recombinant nucleic acid.

The method of recombining a first and a second target nucleic acid, the method comprising:

• providing a first parental nucleic acid sequence and a second parental nucleic acid sequence, wherein the first and second parental nucleic acid sequences are homologous or non-homologous;
• inserting a plurality of insertion nucleic acid sequences into one or more of the first and second parental nucleic acid sequences, thereby providing the first and the second target nucleic acids, wherein the first or second parental nucleic acid sequence hybridizes to a third nucleic acid under stringent conditions, wherein the first and second target nucleic acids do not hybridize under stringent conditions to the third nucleic acid;
• and,
• recombining the first and second target nucleic acids, wherein at least one of the first and second target nucleic acids comprises the plurality of homologous or non-homologous insertion nucleic acid sequences, thereby providing a recombinant nucleic acid.

The method of recombining a first and a second target nucleic acid, the method comprising:

• providing a first and a second target nucleic acid, wherein at least one of the first and second target nucleic acids comprises a plurality of homologous or non-homologous insertion nucleic acid sequences;
• recombining the first and second target nucleic acids, thereby providing a recombinant nucleic acid;
• and,
• recombining the recombinant nucleic acid with a third nucleic acid, and, optionally, selecting the resulting secondary recombinant nucleic acid for a desired trait or property.