Method for producing a synthetic antigen presenting transformed Drosophila cell
AssigneeThe Scripps Research Institute
InventorsZeling Cai, Jonathan Sprent, Anders Brunmark, Michael Jackson, Per A. Peterson
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Abstract
Materials and methods of activating T lymphocytes with specificity for particular antigenic peptides are described, as well as the use of activated T lymphocytes in vitro for the treatment of a variety of disease conditions. In particular, a method for producing a synthetic antigen presenting drosophila cell line for activating T lymphocytes to a specific peptide is described.
Claims2
A method for producing a synthetic antigen presenting transformed Drosophila cell that will specifically activate a T-cell for a peptide comprising:
- a) establishing a culture of Drosophila cells;
- b) transfecting a Drosophila cell with an expressible Class 1 MHC heavy chain gene operably linked to a first promoter;
- c) transfecting said cell with an expressible β-2 microglobulin gene operably linked to a second promoter;
- d) transfecting said cell with an expressible assisting molecule gene operably linked to a third promoter;
- wherein said transformed Drosophila cell expresses functional, Class I MHC heavy chain, β-2 microglobulin and assisting molecule protein on the cell surface;
- and
- e) combining said transformed cell with a peptide;
- wherein said transformed cell and peptide will specifically activate a T-cell specific for said peptide, and said assisting molecule is a costimulatory molecule selected from B7.1 and B7.2.
A method for producing a synthetic antigen presenting transformed Drosophila cell that will specifically activate a T-cell for a peptide comprising:
- a) establishing a culture of Drosophila cells;
- b) transfecting a Drosophila cell with an expressible Class 1 MHC heavy chain gene operably linked to a first promoter;
- c) transfecting said cell with an expressible β-2 microglobulin gene operably linked to a second promoter;
- d) transfecting said cell with an expressible assisting molecule gene operably linked to a third promoter;
- wherein said transformed Drosophila cell expresses functional, Class I MHC heavy chain, β-2 microglobulin and assisting molecule protein on the cell surface;
- and
- e) combining said transformed cell with a peptide;
- wherein said transformed cell and peptide will specifically activate a T-cell specific for said peptide, and said assisting molecule is an adhesion molecule selected from the group consisting of ICAM-1, ICAM-2, ICAM-3 and LFA-3.
Dependencies are inferred from the claim text.