Germination-specific plant promoters
OwnerFinn Lok, Ole Olsen +2inventors
InventorsFinn Lok, Ole Olsen, Per-Johan Meijer, Verena Cameron-Mills
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Abstract
Tissue-specific regulatory sequences, including promoters from barley (Hordeum vulgare) genes encoding α-glucosidase and cystatin-1 (cysteine protease inhibitor) have been identified and isolated. The gene promoters have particular utility in directing high level, germination preferred expression of heterologous proteins that impart increased agronomic and malting characteristics to a given plant. In addition, the invention relates to methods of producing transgenic plants by using chimeric genes, cassette vectors, kits, cells and methods comprising the promoter and signal sequence coding region of the gene for barley α-glucosidase and the gene for barley cystatin-1.
Claims19
An isolated α-glucosidase gene promoter comprising a polynucleotide selected from the group consisting of:
- (a) the nucleic acid sequence of SEQ ID NO:1;
- (b) the nucleic acid sequence of SEQ ID NO:2;
- and
- (c) a fragment of (a) or (b) that directs germination-specific gene expression of an operably linked heterologous nucleic acid sequence.
Dependencies are inferred from the claim text.