US 6,355,861 B1 · Production of gamma linolenic acid by a Δ6-desaturase

Abstract

Linoleic acid is converted into γ-linolenic acid by the enzyme Δ6-desaturase. The present invention is directed to isolated nucleic acids comprising the Δ6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the Δ6-desaturase gene. The present invention provides recombinant constructions comprising the Δ6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.

Claims40

Dependent claims

An isolated nucleic acid encoding an evening primrose Δ6-desaturase.

Dependencies are inferred from the claim text.

Facts

Application

08/934,254Filed 19 Sep 1997 · continuation in part

Priority

10 Oct 1991

Granted

12 Mar 2002

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12N 9/0083
Acting on paired donors with incorporation of molecular oxygen: miscellaneous
C12N 15/74
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12N 15/8247
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified lipid metabolism, e.g. seed…
C12P 7/6427
Fatty acids: polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone

Family

Application07/774,475Parent of this continuation-in-part · filed 10 Oct 1991

Application07/817,919Parent of this continuation-in-part · filed 8 Jan 1992

Application07/959,952Parent of this continuation · filed 13 Oct 1992

Application08/307,382Parent of this continuation-in-part · filed 14 Sep 1994

Application08/789,936Parent of this continuation-in-part · filed 28 Jan 1997

US 5,789,220 AParent of this continuation-in-part

US 5,552,306 AParent of this continuation-in-part

Application08/307,382Parent of this continuation

Application07/959,952Parent of this continuation-in-part

US 6,355,861 B1This patent

From the US filing history. Foreign family members aren’t listed.

Abstract

Facts

Application

08/934,254Filed 19 Sep 1997 · continuation in part

Priority

10 Oct 1991

Granted

12 Mar 2002

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12N 9/0083
Acting on paired donors with incorporation of molecular oxygen: miscellaneous
C12N 15/74
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12N 15/8247
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified lipid metabolism, e.g. seed…
C12P 7/6427
Fatty acids: polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone

Family

Application07/774,475Parent of this continuation-in-part · filed 10 Oct 1991

Application07/817,919Parent of this continuation-in-part · filed 8 Jan 1992

Application07/959,952Parent of this continuation · filed 13 Oct 1992

Application08/307,382Parent of this continuation-in-part · filed 14 Sep 1994

Application08/789,936Parent of this continuation-in-part · filed 28 Jan 1997

US 5,789,220 AParent of this continuation-in-part

US 5,552,306 AParent of this continuation-in-part

Application08/307,382Parent of this continuation

Application07/959,952Parent of this continuation-in-part

US 6,355,861 B1This patent

From the US filing history. Foreign family members aren’t listed.

Production of gamma linolenic acid by a Δ6-desaturase

Life of this patent

Abstract

Claims40

Production of gamma linolenic acid by a Δ6-desaturase

Life of this patent

Abstract

Claims40