Method of detecting a pathogen using a virus
AssigneeHer Majesty the Queen in right of Canada, as represented by the Minister of National Defence
InventorsJohn W. Cherwonogrodzky, Kamil Lotfali
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Abstract
A bacteriophage linked to an enzyme can replace an antibody in a system for detecting the presence of a bacteria in a sample. Specifically Brucella abortus (a pathogen which causes brucellosis in cattle) can be detected using Brucella bacteriophage for the virus, urease for the enzyme linked to the bacteriophage, m-maleimidobenzoyl-N-hydrosysuccimide ester as a coupling reagent, sera from mice immunized with Brucella bacteriophage for a detector antibody, urease conjugated to anti-mouse sheep antibody for an indicator, and urea with bromcresol purple as the substrate. The materials can be used in indirect (sandwich) or direct enzyme-linked viral assays (ELVirA).
Claims4
A conjugate comprising a wild type bacteriophage directly linked to an enzymatically active enzyme.
Dependencies are inferred from the claim text.