Chimeric poxvirus comprising sequences of a retroviral vector component
AssigneeBaxter Vaccine AG
InventorsFalko-Guenter Falkner, Georg Holzer, Friedrich Dorner
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Abstract
A chimeric poxvirus is disclosed, which comprises the sequences of the vector component of a retroviral vector particle and optionally the packaging component of a defective retroviral particle. The chimeric poxvirus according to the invention particularly can be used for applications in gene therapy or tumor therapy.
Claims15
A chimeric poxvirus comprising a poxvirus genome comprising stably inserted therein a replication defective retroviral proviral genome under transcriptional control of a promoter, wherein a foreign gene sequence under transcriptional control of a promoter is inserted into said retroviral genome.
A chimeric poxvirus as set forth in claim 6, wherein said vaccinia virus is a defective vaccinia virus.
A defective retroviral particle comprising an intron-containing genome.
A method for producing a defective retroviral particle comprising a foreign gene sequence, wherein the method comprises
- infecting a cell of a packaging cell line with a chimeric poxvirus comprising a poxvirus genome having stably inserted therein a replication defective retroviral genome under transcriptional control of a promoter, wherein a foreign gene sequence under transcriptional control of a promoter is inserted into said retroviral genome, and wherein the infected cell produces a defective retroviral particle.
A method for producing a defective retroviral particle comprising a foreign gene sequence, wherein the method comprises
- infecting a cell of a cell line with a chimeric poxvirus comprising a poxvirus genome having stably inserted therein a replication defective retroviral genome under transcriptional control of a promoter, wherein a foreign gene sequence under transcriptional control of a promoter is inserted into said retroviral genome and retroviral packaging components under transcriptional control of a promoter, and wherein the infected cell produces a defective retroviral particle.
Dependencies are inferred from the claim text.