# US 6,342,359 B1 · Method for detecting nucleic acids, detector for nucleic acids and method for producing the same

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- Number: US 6,342,359 B1
- Document ID: US6342359B1
- Status: Granted
- Kind: B1
- Application number: 09/672,787
- Filed: 2000-09-29
- Granted: 2002-01-29
- Earliest claimed: 2000-09-29
- Web page: https://patentgrep.com/patent/US6342359B1

## CPC

- C12Q 1/6837: using probe arrays or probe chips (C12Q1/6874 takes precedence)
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- B01J 2219/00576: fluorophore
- B01J 2219/00578: electrophoric
- B01J 2219/00608: DNA chips
- B01J 2219/00612: the surface being inorganic
- B01J 2219/00617: by chemical means
- B01J 2219/0063: Other, e.g. van der Waals forces, hydrogen bonding
- B01J 2219/00635: by reactive plasma treatment
- B01J 2219/00653: the compounds being bound to electrodes embedded in or on the solid supports
- B01J 2219/00677: Ex-situ synthesis followed by deposition on the substrate
- B01J 2219/00704: integrated with the reactor apparatus
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS

## Abstract

The present invention provides a nucleic acid detector for detecting a base sequences of a target DNA of interest, which comprises a DNA chip in which probe DNA and electrochemiluminescent material such as tris(2,2′-bipyridyl) metal complex, or derivatives thereof are immobilized on a surface of gold electrode; an electrochemical apparatus for applying a predetermined voltage to the DNA chip with respect to a reference electrode; and an optical measurement apparatus for measuring electrochemiluminescence generated from the DNA chip. The present invention also provides a method for producing the said detector for nucleic acids, and method for detecting nucleic acids using the same in a cost-saving way with high sensitivity.