US 6,335,161 B1 · Release of intracellular material and the production therefrom of single stranded nucleic acid

Abstract

Intracellular material is released from bacterial, yeast, plant, animal, insect or human cells by the application of a low voltage such as 1 to 10 V to a suspension containing the cells. The conditions may be selected such that DNA released from the cells is electrochemically denatured so as to be available for use in an amplification procedure.

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Facts

Application

09/030,028Filed 25 Feb 1998

Granted

1 Jan 2002

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12N 13/00
Treatment of microorganisms or enzymes with electrical or wave energy
C12N 1/06
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Release of intracellular material and the production therefrom of single stranded nucleic acid

Life of this patent

Abstract

Claims0

Release of intracellular material and the production therefrom of single stranded nucleic acid

Life of this patent

Abstract

Claims0