Chromatographic separation method of paclitaxel and cephalomannin
AssigneeIndena S.p.A.
InventorsBruno Gabetta, Gianfranco Zini
Life of this patent
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Abstract
A method for separating paclitaxel from cephalomannin and other related compounds by obtaining a starting material that contains paclitaxel and cephalomannin; dissolving the starting material in any one of a number of particularly defined solvents such as butyl formate and butyl acetate to form a mixture; subjecting the mixture to column chromatography to obtain an eluted fraction of paclitaxel, an eluted fraction of cephalomannin and a residue; and separately drying the paclitaxel and cephalomannin fractions to obtain separate crystalline forms of paclitaxel and cephalomannin, respectively.
Claims14
A method for separating paclitaxel from cephalomannin which comprises:
- obtaining a starting material that contains paclitaxel and cephalomannin;
- dissolving the starting material in a solvent having the following formula:
- where R1 is hydrogen or methyl and R2 is an alkyl or arylalkyl group containing four to seven carbon atoms, to form a mixture;
- subjecting the mixture to column chromatography to obtain an eluted fraction of paclitaxel, an eluted fraction of cephalomannin and a residue;
- and
- separately drying the paclitaxel and cephalomannin fractions to obtain separate crystalline forms of paclitaxel and cephalomannin, respectively.
A method for separating paclitaxel from cephalomannin which comprises:
- obtaining a starting material that contains paclitaxel and cephalomannin;
- dissolving the starting material in a solvent having the following formula:
- where R1 is hydrogen or methyl and R2 is an alkyl or arylalkyl group containing four to seven carbon atoms, to form a mixture;
- subjecting the mixture to column chromatography utilizing a direct-phase silica gel column with using a single solvent of the formula given above as an elutant to obtain an eluted fraction of paclitaxel, an eluted fraction of cephalomannin and a residue;
- and
- separately drying the paclitaxel and cephalomannin fractions to obtain separate crystalline forms of paclitaxel and cephalomannin, respectively.
Dependencies are inferred from the claim text.