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US 6,333,398 B1Granted
Protein purification
AssigneeGenentech, Inc.
InventorGregory S. Blank
Life of this patent
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Abstract
A method for purifying proteins by Protein A chromatography is described which comprises the steps of: (a) adsorbing the protein to Protein A immobilized on a solid phase comprising silica or glass; (b) removing contaminants bound to the solid phase by washing the solid phase with a hydrophobic electrolyte solvent; and (c) recovering the protein from the solid phase.
Claims15
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase comprising silica or glass;
- (b) removing contaminants bound to the solid phase by washing the solid phase with a hydrophobic electrolyte solvent;
- and
- (c) recovering the protein from the solid phase.
1 more element
Dependencies are inferred from the claim text.