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US 6,333,318 B1Granted
Formulations useful for modulating expression of exogenous genes in mammalian systems, and products related thereto
AssigneeThe Salk Institute for Biological Studies
InventorsRonald M. Evans, Enrique Saez
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Abstract
In accordance with the present invention, there are provided various methods for modulating the expression of an exogenous gene in a mammalian subject employing modified ecdysone receptors. Also provided are modified ecdysone receptors, as well as homomeric and heterodimeric receptors containing same, nucleic acids encoding invention modified ecdysone receptors, modified hormone response elements, gene transfer vectors, recombinant cells, and transgenic animals containing nucleic acids encoding invention modified ecdysone receptor.
Claims14
A formulation comprising:
- at least one ecdysteroid which is an activator for a modified ecdysone receptor,
- at least one RXR or USP agonist which is an activator for a silent partner for said modified ecdysone receptor, and
- a pharmaceutically acceptable carrier;
- wherein said modified ecdysone receptor comprises:
- a ligand binding domain capable of binding an ecdysteroid;
- a DNA-binding domain;
- and
- an activation domain,
- wherein at least one of said DNA-binding domain or said activation domain is not obtained from a native ecdysone receptor wherein said silent partner is RXR or USP, and
- wherein said modified ecdysone receptor forms a heterodimer with said RXR or USP.
7 more elements
A kit comprising:
- at least one ecdysteroid which is an activator for a modified ecdysone receptor,
- at least one RXR or USP agonist which is an activator for a silent partner for said modified ecdysone receptor, and
- a pharmaceutically acceptable carrier;
- wherein said modified ecdysone receptor comprises:
- a ligand binding domain capable of binding an ecdysteroid;
- a DNA-binding domain;
- and
- an activation domain,
- wherein at least one of said DNA-binding domain or said activation domain is not obtained from a native ecdysone receptor wherein said silent partner is RXR or USP, and
- wherein said modified ecdysone receptor forms a heterodimer with said RXR or USP.
7 more elements
Dependencies are inferred from the claim text.