Liposomes containing oligonucleotides
AssigneeGeorgetown University School of Medicine
InventorsUsha Kasid, Prafulla Gokhale, Anatoly Dritschilo, Aquilur Rahman
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Abstract
It is possible to radiosensitize tumor cells by administration of compositions containing the Human antisense c-raf-1 oligodeoxyribonucleotide (ODN/oligo) sequence: 5′-GTGCTCCATTGATGC-3′ (seq. ID No. 1) wherein only the end bases are phosphorylated is a preferred embodiment. Antisense sequences of up to 40 bases which containing this sequence may be used in accord with the teachings of this disclosure. Compositions comprising a cationic liposome of dimethyldioctadecyl ammonium bromide, phosphatidylcholine and cholesterol may be used as a carrier system. The liposomes provide a new carrier system that is particularly useful for administration of sequences for therapy.
Claims7
A method of radiosensitizing tumor tissue by administration of a composition comprising a cationic liposome, phosphatidylcholine and cholesterol, and further comprising a radiosensitizing effective amount of at least one encapsulated antisense oligonucleotide of no more than 40 bases containing the sequence 5′-GTGCTCCATTGATGC-3′ (SEQ ID NO:
- 1).
A composition of matter comprising liposomes consisting essentially of dimethyldioctadecyl ammonium bromide (DDAB), phosphatidylcholine (PC), and cholesterol, and containing the sequence 5′-GTGCTCCATTGATGC-3′ (Seq. ID No:1 wherein only the terminal sequences are phosphorothioated.
Dependencies are inferred from the claim text.