Human-derived glycoprotein, biologically active factor which includes glycoprotein, and pharmaceutical product which comprises biologically active factor as active component

An isolated and purified glycoprotein obtained from the culture broth of human derived fibroblasts and which has the following physicochemical properties,

• a. Molecular weight ranges;
• On the determinations of molecular weight by SDS gel electrophoresis, 76,000-80,000 or 72,000-76,000 under the non-reduced conditions and a common band A with 50,000-54,000 and band B with 28,000-32,000 or band C with 24,000-28,000 under the reduced conditions;
• b. Isoelectric point;
• 7.4 to 8.6;
• c. Heat stability;
• Stable in heating at 60° C. for 10 min;
• d. pH stability;
• Stable in the range of pH6 to 9;
• e. Carbohydrate chain;
• Adsorbed to a Concanavalin A (Con A)-Sepharose column;
• f. Biological activity;
• Inhibits the growth of KB cells, Hela cells, and L-929 cells but not IMR-90 cells;
• g. Reactivity to antibodies;
• The cytotaxic activity is not neutralized by anti-TNF antibody, anti-lymphotoxin antibody, and anti-interferon-β antibody;
• h. N-terminal amino acid sequence;
• Above mentioned band B and band C are subchains of band A, respectively;
• N-terminus of band A is blocked. Band B and band C have commnon N-terminal amino acid sequence as follows;
• Val-Val-Asn-Gly-Ile-Pro-Thr- or
• Val-Val-Asn-Gly-Ile-Pro-Thr-X-Thr-Asn-Ile-Gly-X-Met-Val-Ser-Leu-
• X means an unidentified amino acid.