Method of analyzing bisphenols and polyphenols
AssigneeLaboratory of Molecular Biophotonics
InventorsHitoshi Nohta, Hideyuki Yoshida, Masatoshi Yamaguchi
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Abstract
The method of analyzing polyphenols and bisphenols in accordance with the present invention comprises a step of reacting a bisphenol and/or a polyphenol with a labeling reagent having a pyrene group, so as to generate a fluorescent derivative; a step of irradiating the fluorescent derivative with excitation light and detecting fluorescence emitted from the fluorescent derivative; and a step of calculating an amount of the bisphenol and/or polyphenol in a test sample according to an intensity of thus detected fluorescence and a relationship between a known concentration of a bisphenol and/or a polyphenol contained in a standard sample and fluorescence intensity.
Claims8
A method of analyzing polyphenols and bisphenols for analyzing a polyphenol and/or a bisphenol having at least two phenolic hydroxyl groups contained in a test sample, said method comprising:
- a step of reacting said bisphenol and/or said polyphenol with a labeling reagent having a pyrene group, so as to generate a fluorescent derivative;
- a step of irradiating said fluorescent derivative with excitation light and detecting fluorescence emitted from said fluorescent derivative;
- a step of calculating an amount of said bisphenol and/or said polyphenol in said test sample according to an intensity of thus detected fluorescence and a relationship between a known concentration of a bisphenol and/or a polyphenol contained in a standard sample and fluorescence intensity.
Dependencies are inferred from the claim text.