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AssigneeThe Children's Hospital of Philadelphia
InventorsRobert J. Levy, Timothy Crombleholme, Narendra Vyavahare
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The invention relates to compositions and methods for delivering a virus vector to an animal. The compositions include compositions which comprise a hydrogel matrix (e.g. a collagen matrix which can comprise a poloxamer or an alginate) containing a virus vector therein in a transfectious form. The invention also includes methods of making such hydrogel precursor mixtures and hydrogel matrices, including particles, devices, bulk materials, and other objects which comprise, consist of, or are coated with such mixtures or matrices. The invention further relates to compositions comprising a hydrogel precursor mixture having a virus vector suspended therein, which, when administered to an animal, gel to form a hydrogel matrix containing a virus vector therein in a transfectious form. Methods of delivering a virus vector to an animal tissue are also described.
A composition for delivery of a virus vector to an animal cell by local administration, the composition comprising a hydrogel precursor mixture having the virus vector suspended therein, wherein a component of the hydrogel precursor mixture is bound with an antibody which binds specifically with the virus vector, and wherein the hydrogel precursor mixture stiffens at physiological temperature and at a physiological calcium level to form a hydrogel matrix containing the virus vector therein in a transfectious form.
An implantable device having a surface coated with a hydrogel matrix containing a virus vector therein in a transfectious form, wherein the hydrogel matrix is bound with an antibody which binds specifically with the virus vector.
A method of making a composition for delivery, of a virus vector to an animal tissue, the method comprising administering to a body location in fluid communication with the tissue a hydrogel precursor mixture having the virus vector suspended therein, wherein a component of the hydrogel precursor mixture is bound with an antibody which binds specifically with the virus vector, whereby the hydrogel precursor mixture stiffens upon administration to the body location to form a hydrogel matrix containing the virus vector therein in a transfectious form.
A method of making a composition for delivery of a virus vector to an animal cell, the method comprising contacting the cell with a hydrogel precursor composition which comprises a collagen bound with an antibody which binds specifically with the virus vector, a poloxamer, and the virus vector at physiological temperature and at a physiological concentration of a polyvalent cation, whereby the hydrogel precursor mixture stiffens to form a hydrogel matrix containing the virus vector therein in a transfectious form.
A method of delivering a virus vector to an animal tissue, the method comprising administering to a body location in fluid communication with the animal tissue a hydrogel precursor mixture having the virus vector suspended therein, wherein a component of the hydrogel precursor mixture is bound with an antibody which binds specifically with the virus vector, whereby the hydrogel precursor mixture stiffens upon administration to the body location to form a hydrogel matrix containing the virus vector therein in a transfectious form.
A method of delivering a virus vector to an animal cell, the method comprising placing in fluid communication with the cell a composition comprising a hydrogel matrix having the virus vector contained therein in a transfectious form, wherein a component of the hydrogel precursor mixture is bound with an antibody which binds specifically with the virus vector.
A kit comprising a collagen bound with an antibody which binds specifically with a virus vector, a poloxamer, and an instructional material which describes a method of making a collagen hydrogel matrix which comprises the poloxamer and which contains the virus vector therein in a transfectious form.
Dependencies are inferred from the claim text.