Method of producing an undifferentiated avian cell culture using avian primordial germ cells
AssigneeNorth Carolina State University
InventorsJames N. Petitte, Il-Kuk Chang
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Abstract
A method of producing undifferentiated avian cells expressing an embryonic stem cell phenotype. The method includes the steps of collecting avian gonadal cells comprising primordial germ cells from an avian embryo after the formation of the primitive streak; depositing the avian gonadal cells in contact with a preconditioned feeder matrix; and growing the avian gonadal cells on the pre-conditioned feeder matrix in the presence of media for a time sufficient to produce an avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.
Claims43
A method of producing a sustained culture of undifferentiated avian cells expressing an embryonic stem cell phenotype, comprising:
- (a) collecting avian gonadal cells comprising primordial germ cells from an avian embryo after formation of the primitive streak;
- (b) depositing the avian gonadal cells in contact with a preconditioned feeder matrix;
- and
- (c) growing the avian gonadal cells on the preconditioned feeder matrix in the presence of media for a time sufficient to produce an avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.
A method of producing a sustained culture of undifferentiated avian cells expressing an embryonic stem cell phenotype, comprising:
- (a) collecting avian gonadal cells comprising primordial germ cells from an avian embryo after formation of the primitive streak;
- (b) depositing the avian gonadal cells in contact with a preconditioned fibroblast feeder cell matrix;
- and
- (c) growing the avian gonadal cells on the feeder cell matrix in the presence of media for a time sufficient to produce an avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.
A method of producing a sustained culture of undifferentiated chicken cells expressing an embryonic stem cell phenotype, comprising:
- (a) collecting gonadal cells from a stage 27 to stage 30 chicken embryo;
- (b) depositing the gonadal cells in contact with a preconditioned mouse fibroblast feeder cell layer;
- (c) growing the gonadal cells on the preconditioned mouse fibroblast feeder cell layer in the presence of a conditioned media for a time sufficient to produce a sustained avian cell culture, the sustained avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.
Dependencies are inferred from the claim text.