# US 6,333,184 B1 · Motor proteins and methods for their use

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- Number: US 6,333,184 B1
- Document ID: US6333184B1
- Status: Granted
- Kind: B1
- Application number: 09/718,841
- Filed: 2000-11-22
- Granted: 2001-12-25
- Earliest claimed: 2000-11-22
- Assignees: Cytokinetics, Inc.
- Inventors: Christophe Beraud; Richard Freedman
- Web page: https://patentgrep.com/patent/US6333184B1

## CPC

- C12N 9/14: Hydrolases (3)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention provides isolated nucleic acid and amino acid sequences of HsKif16a, antibodies to HsKif16a, methods of screening for HsKif16a modulators using biologically active HsKif16a, and kits for screening for HsKif16a modulators.

## Claims

### Claim 1

An isolated protein, wherein the protein has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm, wherein said protein comprises a motor domain and said protein has microtubule stimulated ATPase activity.

### Claim 2 (depends on 1)

An isolated protein of claim 1, wherein the protein specifically binds to polyclonal antibodies to HsKif16a, said HSKif16a having an amino acid sequence of SEQ ID NO:2.

### Claim 3

An isolated protein, wherein the protein has an amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4.

### Claim 4 (depends on 1)

An isolated protein of claim 1, wherein the protein specifically binds to polyclonal antibodies generated against a motor domain of HsKif16a, said motor domain having an amino acid sequence of SEQ ID NO:4.

### Claim 5 (depends on 3)

An isolated protein of claim 3, wherein the protein comprises an amino acid sequence of SEQ ID NO:4.

### Claim 6 (depends on 3)

An isolated protein of claim 3, wherein the protein comprises an amino acid sequence of SEQ ID NO:2.

### Claim 7 (depends on 1)

An isolated protein of claim 1, wherein the protein has greater than 80% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.

### Claim 8 (depends on 1)

An isolated protein of claim 1, wherein the protein has greater than 90% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.

### Claim 9 (depends on 1)

An isolated protein of claim 1, wherein the protein has greater than 95% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.