Yield when disulfide-bonded proteins are secreted
AssigneeRoche Diagnostics GmbH
InventorsRudolf Glockshuber, Martina Wunderlich, Arne Skerra, Rainer Rudolph
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Abstract
The invention concerns a process for increasing the formation of the natural protein conformation when disulfide-bonded proteins are secreted by a prokaryotic host organism that contains a recombinant DNA coding for the secreted protein whereby the host organism is cultured in a suitable culture medium under suitable conditions for the expression of the recombinant DNA, which is characterized in that a culture medium containing 0.1 to 20 mmol/l of one or several thiol reagents is used.
Claims28
A process for increasing the formation of the natural protein conformation when disulfide bonded proteins are secreted by an E.coli host that contains a recombinant DNA coding for the secreted protein, comprising culturing the host in a suitable culture medium in the presence of oxygen under conditions suitable for the expression of the recombinant DNA, wherein said culture medium contains 0.1 to 20 mmol/l of one or more thiol reagents.
Dependencies are inferred from the claim text.