Genes and proteins controlling cholesterol synthesis
AssigneeThe Regents of the University of California
InventorsJasper D. Rine, Randolph Hampton
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Abstract
The present invention provides isolated nucleic acid sequences which encode a family of HMG-CoA Reductase Degradation (HRD) polypeptides. More particularly, the present invention provides isolated HRD1, HRD2 and HRD3 nucleic acids and the Hrd polypeptides encoded by such nucleic acids, i.e., Hrd1, Hrd2 and Hrd3, respectively. Vectors comprising the nucleic acids are provided. In addition, the present invention provides screening assay related to cholesterol biosynthesis.
Claims36
An expression vector comprising an expressed gene which hybridizes under stringent conditions to a nucleic acid consisting of SEQ ID NO:1, wherein the expressed gene encodes an HMG-CoA Reductase Degradation (HRD) polypleptide which regulates the degradation of an HMG-CoA reductase, and wherein the stringent hybridization conditions comprise 50% formamide with 1 mg of heparin at 42° C. overnight, followed by a 0.2×SSC wash at 65° C. for 15 minutes.
An expression vector comprising an expressed gene which hybridizes under stringent conditions to a nucleic acid consisting of SEQ ID NO:2, wherein the expressed gene encodes an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase, and wherein the stringent hybridization conditions comprise 50% formamide with 1 mg of heparin at 42° C. overnight, followed by a 0.2×SSC wash at 65° C. for 15 minutes.
An expression vector comprising an expressed gene which hybridizes under stringent conditions to a nucleic acid consisting of SEQ ID NO:5, wherein the expressed gene encodes an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase, and wherein the stringent hybridization conditions comprise 50% formamride with 1 mg of heparin at 42° C. overnight, followed by a 0.2×SSC wash at 65° C. for 15 minutes.
An expression vector comprising an expressed gene which hybridizes under stringent conditions to a nucleic acid consisting of SEQ ID NO:6, wherein the expressed gene encodes an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase, and wherein the stringent hybridization conditions comprise 50% formamide with 1 mg of heparin at 42° C. overnight, followed by a 0.2×SSC wash at 65° C. for 15 minutes.
An expression vector comprising an expressed gene which hybridizes under stringent conditions to a nucleic acid consisting of SEQ ID NO:7, wherein the expressed gene encodes an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase, and wherein the stringent hybridization conditions comprise 50% formamide with 1 mng of heparin at 42° C. overnight, followed by a 0.2×SSC wash at 65° C. for 15 minutes.
An expression vector comprising an expressed gene which encodes a polypeptide comprising a sequence at least 95% identical to SEQ ID NO:3, wherein the polypeptide is an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase.
An expression vector comprising an expressed gene which encodes a polypeptide comprising a sequence at least 95% identical to SEQ ID NO:4, wherein the polypeptide is an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase.
An expression vector comprising an expressed gene which encodes a polypeptide comprising a sequence at least 95% identical to SEQ ID NO:8, wherein the polypeptide is an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase.
An expression vector comprising an expressed gene which encodes a polypeptide comprising a sequence at least 95% identical to SEQ ID NO:25, wherein the polypeptide is an HMG-CoA Reductase Degradation (HRD) polypeptide which regulates the degradation of an HMG-CoA reductase.
Dependencies are inferred from the claim text.