CD4 gene regulatory sequences specifically expressed in mature T cells
AssigneeUniversite Pierre et Marie Curie
InventorsDavid Klatzmann, Patrick Salmon, Olivier Boyer
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Abstract
The present invention relates to compositions and methods for the expression of nucleic acids or polypeptides into mature T lymphocytes. The invention relates more specifically to methods of selective gene expression into mature T lymphocytes, based on CD4-derived regulatory sequences, such as CD4-derived enhancer sequences. The invention is particularly suited for regulating gene expression into mature T lymphocytes in vitro, ex vivo or in vivo, upon genetic modification of hematopoietic precursors and maturation thereof.
Claims10
A method of causing selective expression of a nucleic acid in mature T lymphocytes, comprising:
- (i) providing a vector comprising said nucleic acid operably linked to a CD4 enhancer sequence and a CD4 promoter sequence, wherein the vector comprises, in the 5′→3′ order:
- a sequence of a CD4 enhancer
- a sequence of a CD4 promoter
- the nucleic acid to be expressed, and
- a polyadenylation signal;
- and
- (ii) introducing said vector into hematopoietic cells in vitro or ex vivo, said introduction causing expression of said nucleic acid selectively into T lymphcytes matured from said hematopoietic cells.
Dependencies are inferred from the claim text.