HER2 extracellular domain
OwnerRobert Michael Hudziak, H. Michael Shepard +1inventors
InventorsRobert Michael Hudziak, H. Michael Shepard, Axel Ullrich
Life of this patent
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Abstract
An extracellular portion of the HER2 molecule, essentially free of transmembrane and cytoplasmic portions, which is antigenic in animals. Isolated DNA encoding the extracellular portion; an expression vector containing the isolated DNA; and a cell containing the expression vector. A process for producing the extracellular domain. A vaccine containing the extracellular domain.
Claims16
A process for producing a secreted, glycosylated extracellular domain of the HER2 receptor, comprising the steps of:
- (a) culturing an eukaryotic host comprising DNA encoding the extracellular domain, wherein said DNA terminates 1 base pair to about 24 base pairs upstream of DNA encoding the transmembrane domain of said HER2 receptor, under conditions suitable for expression of said DNA and secretion of said extracellular domain;
- and
- (b) isolating said extracellular domain from said host, wherein said isolated exracellular domain is immunogenic in a human and provokes a humoral and cell-mediated response against HER2 receptor upon administration to a patient.
A method for making a polypeptide fusion comprising an extracellular domain of the HER2 receptor conjugated to a Fc portion of an immunoglobulin molecule, comprising:
- (a) culturing a host cell comprising DNA encoding said polypeptide fusion under conditions suitable for expression of said DNA;
- and
- (b) recovering said polypeptide fusion from said host cell.
A method for making a vaccine useful for Active Specific Immunotherapy, comprising:
- (a) culturing a host cell comprising DNA encoding the extracellular domain of the HER2 receptor or a portion thereof which provokes a humoral and cell-mediated response against HER2 receptor in a patient vaccinated therewith under conditions suitable for expression of said DNA;
- (b) recovering said extracellular domain or portion thereof from said host cell;
- and
- (c) combining said recovered extracellular domain or portion thereof with an adjuvant which is safe and effective in humans so as to generate a vaccine which provokes a humoral and cell-mediated response against HER2 receptor in a patient vaccinated therewith.
Dependencies are inferred from the claim text.