Kainate-binding, human CNS receptors of the EAA5 family
AssigneeNPS Allelix Corp.
InventorsRajender Kamboj, Candace E. Elliott, Stephen L. Nutt
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Abstract
Neurotransmission by excitatory amino acids (EAAs) such as glutamate is mediated via membrane-bound surface receptors. DNA coding for one family of these receptors of the kainate-binding type of EAA receptors, has now been isolated and the receptor protein characterized. Herein described are recombinant cell lines which produce the EAA receptor as a heterologous membrane-bound product. Also described are related aspects of the invention, which are of commercial significance. Included is use of the cell lines as a tool for discovery of compounds which modulate EAA receptor stimulation.
Claims3
A method of assaying a test ligand for the ability to bind to a human CNS receptor, said method comprising the steps of
- (a) incubating a test ligand with:
- a genetically engineered cell that produces a kainate-binding human EAA5 receptor selected from the group consisting of:
- the human EAA5a receptor encoded by residues 1-888 of SEQ ID NO:2;
- the human EAA5b receptor encoded by residues 1-888 of SEQ ID NO:2 wherein residue 321 is arginine;
- and
- the human EAA5c receptor encoded by residues 1-838 of SEQ ID NO:2 followed by two proline residues;
- said cell having incorporated expressibly therein a heterologous DNA molecule encoding a human EAA5 receptor or,
- a membrane preparation derived from said cell, wherein said incubation is performed under conditions which permit binding of said test ligand with said human EAA5 receptor;
- and
- (b) determining the extent of binding between said test ligand and said human EAA5 receptor by comparison with kainate-binding to said EAA5 receptor.
Dependencies are inferred from the claim text.