Disassociation of interacting molecules
AssigneeAffymetrix, Inc.
InventorsDavid N. Miller-Jones, Karin Bergmann, Susan L. Watson
Life of this patent
Estimating the term…
Abstract
dsDNA or other interacting molecules, e.g. antibody and antigen, are disassociated by applying an electrical voltage to a solution containing said molecules in a buffer favoring said disassociation e.g. CHES, CAPS OR CAPSO.
Claims28
A process for denaturing nucleic acid molecules, comprising subjecting a liquid containing said nucleic acid molecules to an electrical voltage applied between electrodes under conditions such as to wholly or partially denature at least a proportion of said nucleic acid molecules in the presence of a buffer selected from the group consisting of N-cyclohexyl-2-aminoethane sulphonic acid (CHES), N-cyclohexyl-3-aminoethane sulphonic acid (CAPS), and N-cyclohexyl-3-amino-2-hydroxypropane sulphonic acid (CAPSO).
A process of repeated denaturation of nucleic acid molecules, wherein said nucleic acid molecules are denatured by a process comprising subjecting a liquid containing said nucleic acid molecules to an electrical voltage applied between electrodes under conditions such as to wholly or partially denature at least a proportion of said nucleic acid molecules in the presence of a buffer selected from the group consisting of N-cyclohexyl-2-aminoethane sulphonic acid (CHES), N-cyclohexyl-3-aminoethane sulphonic acid (CAPS), and N-cyclohexyl-3-amino-2-hydroxypropane sulphonic acid (CAPSO), in which said voltage is applied as a repeating pulse having a duration of up to 2 minutes.
A process of amplifying a target sequence of nucleic acid comprising hybridisation, extension and denaturation of nucleic acid wherein said denaturation is conducted by subjecting said nucleic acid to a voltage applied between electrodes in the presence of a buffer selected from the group consisting of N-cyclohexyl-2-aminoethane sulphonic acid (CHES), N-cyclohexyl-3-aminoethane sulphonic acid (CAPS), and N-cyclohexyl-3-amino-2-hydroxypropane sulphonic acid (CAPSO).
A kit for use in a process of denaturing nucleic acid molecules which comprises an electrode, a counter electrode, optionally a reference electrode, and a buffer selected from the group consisting of N-cyclohexyl-2-aminoethane sulphonic acid (CHES), N-cyclohexyl-3-aminoethane sulphonic acid (CAPS), and N-cyclohexyl-3-amino-2-hydroxypropane sulphonic acid (CAPSO).
Dependencies are inferred from the claim text.