US 6,333,151 B2 · Nucleic acid markers for rice blast resistance genes and rice blast resistance genes isolated by the use of these markers

Abstract

The present invention provides nucleic acid markers for rice blast resistance genes Pi-ta and Pi-ta2, which hybridize with any RFLP probes isolated from rice genomic DNA and are located within a distance of 2.0 cM from rice blast resistance genes Pi-ta or Pi-ta2. According to the present invention, it becomes possible to easily identify rice blast resistance genes and related genes from rice cultivars containing Pi-ta or Pi-ta2, thus promoting development and breeding of superior cultivars. It becomes also possible to easily carry out a resistance test of rice. This will open up way to create new resistance genes.

Claims3

Dependent claims

A method for identifying a rice genomic region of the rice blast resistance gene Pi-ta or Pi-ta2, which comprises:

digesting rice genomic DNA with enzymes selected from the group consisting of Bgl, EcV and Hin, to obtain rice genomic DNA fragments,
hybridizing the rice genomic DNA fragments with RFLP probes selected from the group consisting of XNpb289, XNpb196, XNpb319, XNpb239-1, XNpb154, XNpb88, XNpb261, RUBss and XNpb316,
identifying the rice genomic DNA fragments on which the RFLP probes hybridize as nucleic acid markers for the rice genomic region of the rice blast resistance gene Pi-ta or Pi-ta2, and
identifying the rice genomic region of the rice blast resistance gene Pi-ta or Pi-ta2 with the nucleic acid markers.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

08/282,557Filed 29 Jul 1994

Priority

JP 5-18854529 Jul 1993

Published

US 2001/0002420 A131 May 2001

Granted

25 Dec 2001

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/6895
For detection or identification of organisms: for plants, fungi or algae
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12Q 1/683
For detection of mutation or polymorphism: involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
C12Q 2600/13
Oligonucleotides characterized by their use: plant traits

Nucleic acid markers for rice blast resistance genes and rice blast resistance genes isolated by the use of these markers

Life of this patent

Nucleic acid markers for rice blast resistance genes and rice blast resistance genes isolated by the use of these markers

Life of this patent

Abstract

Claims3